产品货号：
SY0638
中文名称：
β-Tubulin单抗
英文名称：
β-Tubulin, Mouse mAb
产品规格：
40μl|100μl|1ml
发货周期：
1～3天
产品价格：
询价
微管蛋白是一类含有多个成员的蛋白质家族。其最常见的成员是α-微管蛋白和β-微管蛋白，它们是组成微管的主要成分，这两种微管蛋白具有相似的三维结构，能够紧密地结合成二聚体，作为微管组装的亚基。β亚基是由455个氨基酸组成，分子量约为55kDa。本品常作为一种内部参照用于相关免疫学实验。
| 别名 | β-Tubulin,Beta 4 tubulin;TBB5;TUBB2;TUBB2A;tubulin beta 2A |
| SwissProt ID | P07437 |
| 基因ID | 203068 |
| 免疫原 | KLH conjugated synthetic peptide derived from human Beta tubulin: |
| 缓冲液 | 0.01M TBS(pH7.4) with 1% BSA,0.03% Proclin300 and 50% Glycerol. |
| 分子量 | 55KD |
| 克隆类型 | Monoclonal Antibody |
| 亚型 | Mouse IgG1 Monoclonal |
| 宿主 | Rabbit |
| 物种反应 | Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,Monkey,Insect,Yeast. |
| 推荐应用 | IF，WB，IHC，IP,ELISA, |
| 纯化方式 | Affinity Chromatography |
| 细胞定位 | 细胞质，细胞骨架 |
| 研究领域 | 细胞生物学与发育生物学 |
| 推荐应用 | WB | ELISA | IHC-P | IHC-F | ICC | IF |
| 稀释比例 | 1:5000～20000 | 1:5000～10000 | 1:100～500 | 1:100～500 | 1:100 | 1:200～500 |
| 组分 | 40μL | 100μL | 1mL |
| β-Tubulin单抗 | 40μL | 100μL | 1mL |
| 说明书 | 1份 | ||
保存：-20℃，避免反复冻融。
| Western blot | |
 | Western blot analysis of these sample Lane 1:Mouse Cerebrum tissue lysates Lane 2:Rat Cerebrum tissue lysates Lane 3:Rat Cerebellum tissue lysates Lane 4:Human Hela cell lysates Lane 5:Human SH-SY5Y cell lysates Lane 6:Human Jurkat cell lysates Lane 7:Human Huvec cell lysates using beta tubulin antibody at 1/20000 dilution |
 | Western blot analysis of Hela Lysate using Beta tubulin at antibody 1/2000~1/20000 dilution. |
 | Western blot analysis of Cerebrum Lysate using Beta tubulin antibody at 1/1000~1/20000 dilution. |
 | Western blot analysis of Hela，NIH/3T3，HL60 Cerebellum，Cerebrum using beta tubulin antibody at 1/1000 dilution. |
| IHC | |
 | IHC analysis of Paraformaldehyde fixed,paraffin- embedded (rat kidney);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min;Block endogenous peroxidase by 3% hydrogen peroxide for 20minutes;Blocking buffer (normal goat serum) at 37℃ for 30min;Antibody incubation with beta tubulin antibody. |
 | IHC analysis of Paraformaldehyde fixed,paraffin embedded (Mouse brain);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min;Block endogenous peroxidase by 3% hydrogen peroxide for 20minutes;Blocking buffer (normal goat serum) at 37℃ for 30min;Antibody incubation with beta tubulin Antibody. |
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